Journal: Nature Communications

Article Title: GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein

doi: 10.1038/s41467-021-22297-8

Figure Lengend Snippet: a , b Immunofluorescence analysis of N protein in A549-hACE2-Flag cells infected with SARS-CoV-2 for 24 h ( a ). The percentage of cells with N protein foci was quantified, n = 8 biologically independent samples, 20 randomly selected views were analyzed in each sample ( b ). Scale bar, 10 μm. c 3D images were obtained by Zeiss LSM 880 confocal microscope and reconstituted by Volocity 6.1.1 . d , e The inhibitory effect of GCG on the replication of SARS-CoV-2, n = 6 biologically independent samples ( d ). IC 50 was calculated, n = 5 biologically independent samples ( e ). The infection was performed after 1-h pretreatment of GCG. f , g Representative immunofluorescent images showed the inhibitory effect of GCG on SARS-CoV-2 N protein. 3D images were obtained by Zeiss LSM 880 confocal microscope and reconstituted by Volocity 6.1.1 ( f ). Violin plots showing foci of cells ( n = 50 biologically independent cells) from each group, lines within the plots, with 25th, 50th, and 75th percentiles marked ( g ). h Cells were infected with SARS-CoV-2 for 1 h followed by 24-h GCG treatment, n = 3 biologically independent samples. Representative images were shown. SARS-CoV-2 was used at an MOI of 1. Hoechst (blue), nuclear staining ( a , c , f ). Error bars, mean with s.d. ( b , d , e , g , h ). Two-tailed unpaired Student’s t -test, * P < 0.05, **** P < 0.0001. Data are representative of at least three independent experiments. Source data are provided as a Source Data file.

Article Snippet: Images were acquired using Zeiss LSM 880 confocal microscope or DeltaVision Deconvolution microscope.

Techniques: Immunofluorescence, Infection, Microscopy, Staining, Two Tailed Test